Scores of autoimmune diseases afflicting one in 12 Americans — ranging from type 1 diabetes, to multiple sclerosis (MS), to rheumatoid arthritis, to asthma — mysteriously cause the immune system to harm tissues within our own bodies. Now, a new study pinpoints the complex genetic origins for many of these diseases, a discovery that may lead to better diagnosis and ultimately to improved treatments.
A team of scientists from UC San Francisco, the Broad Institute of MIT and Harvard, and Yale School of Medicine developed a new mathematical tool to more deeply probe existing DNA databases. In so doing they discovered how certain DNA variations, when inherited, are likely to contribute to disease.
By applying their method to analyzing data from previous studies of 21 different autoimmune diseases, the research team has deepened scientific understanding of the genetic underpinnings of a wide range of these disorders. They also found the specific immune cells most responsible for the diseases. Their study is published online on October 29, 2014 in Nature.
The researchers examined a wealth of data from 39 large-scale studies called genome-wide association studies (GWAS). Teams of scientists in recent years have conducted GWAS — typically enlisting thousands of study participants — to identify large blocks of DNA within the human genome within which genetic variants are implicated as risk factors for common diseases. But examination of GWAS data to date has seldom pointed to altered proteins, as surprisingly few protein-encoding gene variants within these broad swaths of DNA have been associated with the diseases under investigation.
Instead, the genetic risks identified through GWAS more often appear to be associated with DNA variations that do not reside within genes. The nature of this risk has defied understanding until now, fueling a perception that few medical benefits have thus far emerged from large-scale studies of human genetic variation being conducted in the wake of the initial Human Genome Project.
In the new study the researchers found that the presence of specific genetic variants in different autoimmune diseases can alter patterns of activity of genes in particular ways that affect functions of the immune system. This was true despite the fact that the genetic variants are not within genes.
To make their discoveries, the researchers developed software and used next-generation sequencing techniques to probe “epigenetic” characteristics of specialized immune cells, in which gene activity is affected without changes to the DNA sequence itself within the affected genes.
The team discovered that a majority of key DNA changes associated with autoimmune diseases occur in functional bits of DNA known as “enhancers.”
Although DNA exists within cells as long, stringy molecules, DNA can bend back upon itself with the support of the chromosome’s structural proteins, so that one piece of DNA may interact with another. Enhancers fold in this way to bind to DNA switches that turn genes on. In general the enhancers identified in the Nature study as playing a role in autoimmune disease were DNA sequences that did not match DNA-sequence motifs previously thought to be essential to enhancers, and had not previously been seen as having any functional role.
“Once again, research is revealing new meaning in the world of DNA once thought of as junk — short, seemingly random DNA sequences that in fact serve meaningful roles in human physiology,” said Alex Marson, MD, PhD, UCSF Sandler Faculty Fellow and the corresponding author for the study.
By painstakingly mapping enhancers in specialized immune cells, and by tracking down patterns of altered gene activation that resulted from the presence of variants found in GWAS studies, the researchers identified patterns of activity within the genome and cell types associated with the autoimmune diseases. Many autoimmune diseases were associated with immune cells known as T helpers. The authors suggest that genetic variation may be tuning the response of these key immune cells to stimuli within their surroundings to increase the risk of autoimmunity.
Marson initiated the scientific collaboration while he was an MD/PhD student at Harvard and MIT and a medical resident at the Brigham and Women’s Hospital, joining together with the senior scientists for the study: Bradley Bernstein, MD, PhD, associate member of Broad Institute and professor of pathology at Harvard, and David A. Hafler, MD, professor of neurology and immunobiology, and chair of the Department of Neurology at Yale. Marson and Kyle Kai-How Farh, MD, PhD, a clinical geneticist and postdoctoral fellow with the Broad Institute, are co-first authors of the Nature article.
Among other revelations, the new study strongly links the cause of MS to the immune system, not to genetic variants associated with the nervous system. According to Hafler, the results provide definitive evidence that MS is an autoimmune disease, and that the immune system plays the primary role. “This is highly consistent with the new multiple sclerosis treatments that work on the immune system, suggesting that we finally have a good handle as to the underlying causes of MS,” Hafler said.
The new ability to associate specific genetic variants on one hand with cell circuits that control gene activity and alter the physiology of specific immune cell types on the other will enable medical researchers to more precisely target therapeutic interventions in autoimmune diseases in order to dampen aberrantly fired-up immune responses, according to Marson.
In his UCSF lab, Marson intends to probe more deeply how these newly identified DNA variants in enhancers affect cells, and how their disease-causing effects might be mitigated by DNA manipulations carried out using gene-editing technologies known as CRISPR.
The National Institutes of Health and the National Multiple Sclerosis Society provided major funding for the study.
Story Source:
The above story is based on materials provided by University of California, San Francisco (UCSF). The original article was written by Jeffrey Norris. Note: Materials may be edited for content and length.
Journal Reference:
- Kyle Kai-How Farh, Alexander Marson, Jiang Zhu, Markus Kleinewietfeld, William J. Housley, et al. Genetic and epigenetic fine mapping of causal autoimmune disease variants. Nature, 2014 DOI: 10.1038/nature13835